Four of the bodies were found in the living room and two others in the bedroom.
Police said that two of the victims appeared to have tried to make it to the door, but collapsed and died.
Hotel records showed there were no other visitors to the room.
Police Lieutenant General Trairong Phiwpan, head of the Thai police force’s forensic division, told a news conference on Wednesday one of the six is believed to be the one who committed the murders.
“After staff brought teacups and two hot water bottles, milk and teapots… one of the six introduced cyanide,” he said.
Interviews with relatives of the dead revealed there had been a dispute over debt, police added.
It was also said there could have been a conflict among the six regarding investments worth millions.
The three dead women have been named as 46-year-old Nguyen Thi Phuong, Sherine Chong, 56, and Nguyen Thi Phuong Lan, also 46.
The male victims are Tran Dinh Phu, 37, Dang Hung Van, 55, and 49-year-old Hong Thanh Pham.
The six were last seen alive when the meal was delivered to the room on Monday afternoon.
The Vietnamese government said its embassy in Bangkok was working with Thai authorities on the case, while the US State Department said it was monitoring the situation.
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